We have proposed a nanowell array-based nanobiosensor with high sensitivity, and demonstrated its feasibility using a new electrochemical assay method for single biomolecule assay. We would like to continue to work on this nanowell array-based single biomolecule assay. And establish a firm academic basis for such applications.     To achieve digital monitoring of molecule diagnostics, it is important to develop a nanobiosensor with ultra-high signaling accuracy. To achieve enough sensitivity, we have to solve some critical issues, such as (1) how to immobilize the targeting molecules such as ligand or analyte in a specific positions, (2) how to stabilize targeting molecules and localize biomolecules selectively without non-specific adsorption, and (3) how to detect and quantitatively measure very small that result from the interaction of a single molecules.   we will focus on developing a smart single molecule analysis method using a nanowell array system for better accuracy and more reproducible medicine that can analyze quantitative biomolecule interaction. our proposed nanowell array-based electrochemical analysis method for functional diagnostics of molecules is the instrumental platform for single biomolecule medical care, an example of which includes early diagnostics of the dynamic analysis of a drug. we will also elucidate the mechanism of minute bio-electric signal transfer by the electrochemical characteristics of a one-dimensional interface between electrodes and bio-molecules and of the three dimensional structure of biomolecules.