Recently, much attention has been paid to nano-toxicology, which concerns the safety and hazardous nature of nanomaterials. Careful evaluations of nanoparticles(NPs)-associated toxicity are essential for future advances in nanobiotechnology. The goal of this research is to clarify the physical and chemical properties of the ingredients and to formulate solutions that evaluate and warrant safety to human health. To carry out this research, we will investigate the properties of numerous physiochemically modified nanomaterials on a multiflex microarray-cells-chip (i.e., epidermal cell, derma cell, brain cell, lung cell) Radical oxygen is known to be involved in the pathomechanisms of various disorders. We intend to continue working on a nano point-of-care (POC) chip device for skin radicals, in which a nano-well structure for the diagnostics of a single biomolecule is applied for the direct, real-time, digitalized quantitative measurement of concentrations of skin radicals. Aiming for commercialization in the medical industry, radical generation on the human skin surface will be investigated under controlled measurement conditions, so that the measured values can be referred for pathophysiological implications.