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Eno E. Ebong

Associate Professor,  Chemical Engineering
Affiliated Faculty,  Bioengineering

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Office

  • 221 ISEC
  • 617.373.8744

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Research Focus

Study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis

About

Joined the Chemical Engineering Department in Fall 2013.

The primary focus of our research is to study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis. We are applying engineering to study the structure and function of the endothelial cell surface glycocalyx that directly interfaces with flowing blood and sheds in the presence of atherosclerosis. We are using cryopreservation (rapid freezing/freeze substitution) and transmission electron microscopy to define the ultrastructure of the endothelial surface glycocalyx and its changes as a result of the macro- or micro-vessel origin and due to the bio-chemical and -mechanical environment. RNA interference techniques, fluorescent intracellular biomarkers, fluorescence confocal microscopy, and protein biochemistry are applied to further clarify the mechanisms by which various flow patterns impact endothelial cell surface glycocalyx ultrastructure, its transduction of fluid forces into biological responses, and its role in vascular health or disease. In vivo studies are performed using high fat fed apolipoprotein E (ApoE) knockout mice, a well-established animal model of atherosclerosis, to determine which glycocalyx components can be targeted to prevent, diagnose, or treat atherosclerosis.

Education

  • B.S. (Mechanical Engineering) Massachusetts Institute of Technology, 1999
  • M.Eng. (Biomedical Engineering) Rensselaer Polytechnic Institute, 2001
  • Ph.D. (Biomedical Engineering) Rensselaer Polytechnic Institute, 2006
  • Postdoctoral Fellowship Albert Einstein College of Medicine and CUNY City College of New York, 2007-2012

Honors & Awards

  • NIH Mentored Research Scientist Career Development Award

Research Overview

Study the means by which endothelial cell mechanotransduction occurs in order to prevent or promote atherosclerosis

Department Research Areas

Selected Publications

  • M.J. Cheng, P. Prabakaran, R. Kumar, S. Sridhar, E.E. Ebong. Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery. Journal of Visualized Experiments. 2018 Jan; (131), e56760
  • R. Mitra, G.L. O’Neil, I.C. Harding, M.J. Cheng, S.A. Mensah, E.E. Ebong. Glycocalyx in Atherosclerosis-Relevant Endothelium Function and as a Therapeutic Target. Current Atherosclerosis Reports. 2017 Nov 10; 19(12): 63
  • S.A. Mensah, M.J. Cheng, H. Homayoni, B.D. Plouffe, A.J. Coury, E.E. Ebong. Regeneration of glycocalyx by heparan sulfate and sphingosine 1-phosphate restores inter-endothelial communication. PLoS One. 2017 Oct 12;12(10):e0186116
  • S. Russell-Puleri, N.G. dela Paz, D. Adams,  M. Chattopadhyay, L.E. Cancel, E. Ebong, A.W. Orr, J.A. Frangos, J.M. Tarbell. Fluid shear stress induces upregulation of COX-2 and PGI(2) release in endothelial cells via a pathway involving PECAM-1, PI3K, FAK, and p38. American Journal of Physiology – Heart and Circulatory Physiology. 2017 Mar 1; 312 (3): H485-H500
  • W.T. Wong, S. Ma, X.Y. Tian, A.B. Gonzalez, E.E. Ebong, H. Shen. Targeted Delivery of Shear Stress-Inducible Micrornas by Nanoparticles to Prevent Vulnerable Atherosclerotic Lesions. Methodist Debakey Cardiovasc J. 2016 Sep;12(3):152-156
  • L.M. Cancel*, E.E. Ebong*, S. Mensah, C. Hirschberg, J.M. Tarbell. Endothelial Glycocalyx, Apoptosis and Inflammation in an Atherosclerotic Mouse Model. Atherosclerosis. Atherosclerosis. 2016 Sep; 252:136-46 (* authors made equal contributions to this work)
  • M. Cheng, R. Kumar, S. Sridhar, T.J. Webster, E.E. Ebong. Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting. International Journal of Nanomedicine. 2016 Jul 21;11: 3305-15
  • E. Ebong, S.V. Lopez-Quintero, V. Rizzo, D.C. Spray, J.M.Tarbell, Shear-induced Endothelial NOS Activation and Remodeling via Heparin Sulfate, Glypican-1, and Syndecan-1, Integrative Biology: Quantitative Biosciences from Nano to Macro, 6(3), 2014, 338-347
  • M. Thi, E. Ebong, D. Spray, S. Suadicani, Interaction of the Glycocalyx with the Actin Cytoskeleton, Neuromethods, Springer Publishing, 79, 2013, 43-62
  • E. Ebong, N. Depaola, Specificity in the Participation Of Connexin Proteins in Flow-Induced Endothelial gap Junction Communication, European Journal of Physiology, 465(9), 2013, 1293-302
  • Y. Zeng, E. Ebong, B. Fu, J. Tarbell, The Structural Stability of the Endothelial Glycocalyx after Enzymatic Removal of Glycosaminoglycans, PLoS ONE, 7(8), 2012, e43168
  • E. Ebong, F. Macaluso, D. Spray, J. Tarbell, Imaging the Endothelial Glycocalyx In Vitro by Rapid Freezing/Freeze Substitution Transmission Electron Microscopy, Arteriosclerosis Thrombosis and Vascular Biology, 31(8), 2011, 1908-1915
  • E. Ebong, F. Macaluso, D. Spray, J. Tarbell, Life-like Preservation and TEM Visualization of the Glycocalyx Reveals that it is Substantial in Vitro, Proceedings of the 2011 IEEE 37th Annual Northeast Bioengineering Conference, 2011, 1-2

Faculty

Feb 13, 2019

Ebong Receives NSF CAREER Award

ChE Assistant Professor Eno Ebong received an NSF CAREER award for “EMBRACE STEM (Endothelial MechanoBiology Research And multiCultural Education in STEM).”

Faculty

Dec 19, 2018

Ebong Lab Accomplishments

This semester has been a great one for Chemical Engineering Professor Eno Ebong's Lab.  They have had three accepted papers in the Translational Medicine Communications, International Journal of Nanomedicine, and Journal of Translational Medicine. While the other two are still in press, be sure to take a chance to read "The comparative effects of high fat diet or […]

Students

Mar 26, 2018

PhD Student Selected to Speak at Global Health & Innovation Conference

BioE PhD student Solomon Mensah and CEO of Therapeutic Innovation was selected through a competitive process to present his Social Impact Pitch on “Re-Examining the Design of the Neonatal Bubble-CPAP for Application in the Developing World” at the 15th annual Global Health & Innovation Conference.

Faculty

Jul 24, 2017

Northeastern Researchers Contribute to Methodist DeBakey Cardiovascular Journal

The Methodist DeBakey Cardiovascular Journal (MDCVJ) reports on leading edge research, diagnosis, and treatments from specialists at the Houston Methodist DeBakey Heart & Vascular Center and other invited contributors. In Volume 12, Number 3 (July-September 2016), numerous faculty and one PhD student had their research featured in the journal. Nucleic Acid Delivery for Endothelial Dysfunction in […]

Sep 19, 2016

Eno Ebong, Abby Koppes, & Ryan Koppes to Participate in Tufts CTSI Junior Faculty Career Development Forum

Eno Ebong, Prof. Abby Koppes, and Prof. Ryan Koppes were selected as three out of the 29 junior faculty members chosen to participate in the Tufts CTSI 2016-2017 Junior Faculty Research Career Development Forum.

Graduate

Apr 04, 2016

BioE PhD Student Solomon Mensah Receives 2016 NSF Graduate Research Fellowship Award

Solomon Mensah is a Doctoral student in the Department of Bioengineering and a 2016 recipient of the prestigious National Science Foundation Graduate Research Fellowship Award. He will use his award to investigate inter-cellular interactions in cancer and cardiovascular diseases, and to study the role played by the blood vessel wall sugar coating called “glycocalyx."  Mensah's work, is supervised by Professor […]

Mar 22, 2016

FY17 TIER 1 Award Recipients

23 COE faculty and affiliates were recipients of FY17 TIER 1 Interdisciplinary Research Seed Grants for 12 different projects representing over $600K dollars of investment in research.

Mar 03, 2016

ChE Professor Eno Ebong Presents Invited Talk on “Biomechanics of Vascular Disease" at Tufts University

ChE Assistant Professor Eno Ebong presented an invited talk on “Biomechanics of Vascular Disease" at Tufts University.

Sep 18, 2015

Interdisciplinary Approach to Treat Vascular Diseases

ChE Assistant Professor Eno Ebong is using interdisciplinary research to search for the cause and treat­ment of vas­cular dis­eases that cause heart attacks and strokes.

Sep 09, 2015

Ebong Receives Prestigious NIH Award

ChE Assistant Professor Eno Ebong was awarded the prestigious NIH Mentored Research Scientist Career Development Award.

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